Figure 7 | Scientific Reports

Figure 7

From: Spatiotemporal expression of thyroid hormone transporter MCT8 and THRA mRNA in human cerebral organoids recapitulating first trimester cortex development

Figure 7

Real-time qPCR quantification of acute T3 treatment effects on gene expression. 24-day-old (A) and 44-day-old organoids (B) derived from three individual hiPSC lines were treated for 48 h with 50 nM T3. Total RNA was isolated from pools of 2–3 organoids and effects of T3 treatment on expression of known T3 response genes (KLF9, DBP, FLYWCH2, CADM2), SLC16A2 and THRA as well as cell type markers (SOX2, EOMES, MAP2) were determined by RT-qPCR. Colored dots represent mean expression values determined for organoids of individual hiPSC lines. Fold changes relative to the mean of the control group are shown. Unpaired t-test was performed to detect significant differences between treatments using mean values determined for individual hiPSC lines as statistical units (N = 3). Asterisks denote significant differences between treatment means. ***P < 0.001, **P < 0.01, *P < 0.05. ns, not significant.

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