Figure 3 | Scientific Reports

Figure 3

From: Ginsenoside Rg3, enriched in red ginseng extract, improves lipopolysaccharides-induced suppression of brown and beige adipose thermogenesis with mitochondrial activation

Figure 3

Rg3 suppresses early phases of adipogenesis. 3T3-L1 cells were seeded the day before differentiation and induced to differentiate 2 days later. Rg3 or DMSO (vehicle control) was treated during adipogenesis. (A) Structure of Rg3; The culture of 3T3-L1 cells was treated with 0–60 μM of Rg3. (B) Cell viability (% of control); 3T3-L1 cells were seeded and induced to differentiation in the presence or absence of Rg3 (0–60 μM) for 7–8 d. Triglyceride (TG) accumulation was visualized by Oil-red O (ORO) staining; (C) ORO quantification (OD 500 nm); (D) Representative images from separate experiments of ORO staining (magnification, 4×, down); (E) Adipogenic gene expression of Pparγ, Ap2, and C/ebpα by real-time PCR; (F) Western blot analysis of the adipogenic proteins PPARγ, aP2 and β -actin; (G) Relative mRNA expression levels of Pgc1α, Sirt1, and Nrf2; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 compared to the vehicle (Veh) control using Student’s t-test or one-way ANOVA with Bonferroni’s comparison test.

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