Figure 6 | Scientific Reports

Figure 6

From: Ginsenoside Rg3, enriched in red ginseng extract, improves lipopolysaccharides-induced suppression of brown and beige adipose thermogenesis with mitochondrial activation

Figure 6

Rg3 reversed lipopolysaccharide (LPS)-induced brown thermogenesis with marginal induction of mitochondrial respiration. (A) Brown adipose tissue (BAT) sections stained using hematoxylin and eosin (magnification, 20×; scale bars = 100 µm); (B) Immunoblots of UCP1, and PGC1α in BAT; (C) RT-PCR of the relative gene expression levels of Il-6, and Il-1β in BAT; (D) The experimental scheme for isolation of SubQ fat-derived mesenchymal stem cells (MSCs). Primary brown adipocytes were prepared from brown adipose tissue of C57BL/6 mice. BAT MSCs were induced to differentiate with or without 60 μM of Rg3 for 48 h in the presence of LPS (100 ng/mL) for 72 h; (E) Oxygen consumption rate (OCR) was determined using a Seahorse extracellular analyzer; (F–G) OCR profiles and area under the curve analysis from (E); Data are expressed as mean ± SEM (n = 2–6) and analyzed using one-way ANOVA with Bonferroni's comparison test (CL + LPS vs CL + LPS + Rg3).

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