Figure 1

Outlines of the experimental procedures. (a) Flocculated biomass derived from the co-existence of Synechocystis and fungi was found in a Synechocystis culture under 10 μM EM treatment with a natural fungal contamination. (b) The flocculated biomass from (a) was streaked onto agar medium to isolate the fungi. (c) Co-cultivation between Synechocystis and the isolated fungi obtained from (b). This cultivation was optimized for the maximal level of flocculated biomass with respect to the three indicated factors. (d) The optimal co-cultivation yielding the maximal flocculated biomass was subjected to fungal classification by fungal ITS rDNA sequencing, Synechocystis transcriptomic analysis, and microscopic examination.