Figure 4

Effect of initial Synechocystis density on the levels of Synechocystis-fungi flocculation. Total biomass is the sum of flocculated and suspended biomasses. Values are shown as the mean ± 1SD (n = 4), and ND = not detected. (a) Axenic cultivation of various initial Synechocystis densities in the presence of 10 µM EM. Significantly different levels (*P < 0.05: two-tailed student’s t-test), compared to that of the initial Synechocystis inoculation at OD730nm = 0.1 of the same cultivation time and the same type of biomass. (b) Co-cultivation between various Synechocystis densities and the fungi (fixed initial fungi inoculation of 5 mg fresh weight/100 mL) in the presence of 10 µM EM. Significantly different levels (*P < 0.05: two-tailed student’s t-test) compared to that of the initial Synechocystis inoculation at OD730nm = 0.1 at the same cultivation time and the same type of biomass. Representative images of the cell flocculation from the respective cultivation are shown.