Figure 6

Synechocystis responsive genes in the flocculated cells. (a) Synechocystis responsive genes under flocculation. Synechocystis transcriptomics were compared between Synechocystis in [the Synechocystis-fungi flocculated biomass with EM] and [axenic suspended Synechocystis without EM]. The Synechocystis-fungi flocculated biomass was obtained from the co-cultivation between Synechocystis (initial density of OD₇₃₀ = 0.2) and the fungi (initial inoculation of 5 mg fresh weight in 100 mL cultivation) in the presence of 10 µM EM. Axenic Synechocystis biomass was derived from the axenic culture using an initial density at OD₇₃₀ = 0.2 without EM. The cultivation time is seven days. Data were obtained from three independent cultures. The transcriptomic data were analyzed, and the responsive genes were categorized by their cellular functions and metabolism using the Kyoto Encyclopedia of Genes and Genomes (KEGG; www.genome.jp/kegg/pathway.html)⁷⁰. Names of the genes in each category of cellular processes are given in Supplementary Data S1. (b) Synechocystis-responsive genes under flocculation found in this study (shown in a) have also been reported to respond to other abiotic stresses: hydrogen peroxide⁷¹, copper⁷², cadmium⁷³, nitrogen deprivation⁴⁴, salt⁷⁴, and heat⁷⁵. Names of responsive genes in each stress are given in Supplementary Data S2.