Table 1 Primers used for this study.
Primer Name | Sequence (5′ to 3′) | Annotation |
|---|---|---|
Ddcpl-qF | GATTGTTCTGCTGCCTAC | RT-qPCR |
Ddcpl-qR | AGTGTCATCTGCTCCTAC | |
tba-qF | ACATTCTTCAGTGAGACGCA | RT-qPCR |
tba-qR | ACCTTGGAGACCGTGACATT | |
act-qF | AGGTTGCCGCTTTGGTCG | RT-qPCR |
act-qR | CTTCTGTCCCATTCCGACCA | |
Ddcpl-cF | ATGTGGAAGCGAATGCTG | cDNA cloning |
Ddcpl-cR | TTAGACGAGCGGATAGCT | |
ISH-S | GGTACTCGGAGAACGGCA | In situ hybridization |
ISH-A | GTGACCTCTGCAAGCAG | |
dsDdcpl-F | TAATACGACTCACTATAGGG GTGACCTCTGCAAGCAG | dsRNA synthesis |
dsDdcpl-R | TAATACGACTCACTATAGGG GGTACTCGGAGAACGGCA | |
dsGFP-F | TAATACGACTCACTATAGGG GAGTGCCATGCCCGAAGGT | dsRNA synthesis |
dsGFP-R | TAATACGACTCACTATAGGG GGTCTGCTAGTTGAACGCT |
