Figure 1
From: Sphingosine-1-phosphate receptor 1/5 selective agonist alleviates ocular vascular pathologies
ASP4058 functions as an S1P1 agonist in HRMEC. ASP4058, an S1P1 and S1P5 selective agonist, exerts agonistic effects on HRMEC via S1P1. (A) RT-PCR analysis showing all S1P receptor subtypes mRNA expression except for S1P5 in HRMECs. (B) Flow cytometry analysis showing the S1P1 cell surface expression in HRMECs. Data are shown as a histogram overlay: isotype control-stained cells (green) and anti-S1P1 antibody-stained cells (red). (C) The representative immunofluorescence image of S1P1 receptor (red) and DAPI (blue) of HRMECs. Scale bar = 30 µm. (D) Chemical structure of ASP4058. (E) ASP4058 inhibited forskolin-induced cAMP accumulation in HRMECs. Data are presented as the mean ± SEM (n = 5).
