Figure 1 | Scientific Reports

Figure 1

From: Droplet based whole genome amplification for sequencing minute amounts of purified Mycobacterium tuberculosis DNA

Figure 1

Overview of study design and experimental procedures. High-quality purified DNA (364 ng/μl) extracted from a Lineage 2 Mtb clinical isolate served as a positive control (Illumina MiniSeq WGS). The DNA was diluted to be used as starting material for four dMDA reactions (5 pg, 1 pg, 0.5 pg, and 0.1 pg, respectively). The DNA after dMDA was quantified using a Quantus fluorometer, assessed for fragmentation using the TapeStation™, and subsequently used for library preparation and Illumina WGS on an iSeq instrument. Data analysis was done using the MAGMA pipeline. ONT WGS was done on the control and 5 pg DNA input dMDA sample and the ONT data analysis was done using TB-profiler. Figure created with BioRender.com. Abbreviations: dMDA = droplet multiple displacement amplification; WGA = whole genome amplification; WGS = whole genome sequencing.

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