Figure 3
SMF promoted osteoclast formation by PDLSCs under mechanical stress. (A) Schematic diagram of the in-vitro models. (B) Morphology of PDLSCs stimulated by mechanical loading and SMF after 24 h. (C) Schematic diagram of osteoclast formation induced by the PDLSCs supernatant. (D,E) Representative TRAP images showing osteoclast formation after 7 days of induction. (F,G) Western blot image and semi-quantitative measurement of TRAP expression in osteoclasts after 7 days of induction. n = 3; ns, not significant; **P < 0.01 vs Con group; #P < 0.05 vs Mech. loading group.
