Figure 5 | Scientific Reports

Figure 5

From: Static magnetic field-induced IL-6 secretion in periodontal ligament stem cells accelerates orthodontic tooth movement

Figure 5

SMF promoted IL-6 secretion from PDLSCs under mechanical stress. (A) RT-PCR assay of expression levels of IL-1β, TNF-α, IL-6, RANKL, OPG, and RANKL/OPG relative to GAPDH in PDLSCs after 24 h. Data for each gene was processed using the 2ΔΔCt method. (B) ELISA of IL-6 protein in PDLSCs supernatant after 24 h. (C) RT-PCR was performed to evaluate the efficiency of IL-6 inhibition (*P < 0.05). (D,E) Representative TRAP images showing osteoclast formation after seven days of induction. (F,G) Immunohistochemistry of IL-6 expression levels on the pressure side of tooth movement. AOD integrated optical density/area; n = 3; data are mean ± SD; *P < 0.05 vs Con; #P < 0.05 vs OTM + sham.

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