Figure 10

Heat stress-induced transcriptomic alterations together with acute phase response ensued in neural, hepatic, and systemic inflammation debilitating synaptic plasticity. (A) IL-6, BDNF, and TSPO immunoreactivities were shown by immunohistochemistry in the brain (frontal cortex) sections from Control and Heat Stress mouse groups; Images were taken at 40X magnification displayed with the scale of 50 μm. (B) IL-6 immunoreactivity in both groups was measured as arbitrary light units from six separate microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. (C) BDNF immunoreactivity in both groups was measured as arbitrary light units from six separate microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. (D) TSPO immunoreactivity in both groups was measured as arbitrary light units from six separate microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. (E) Serum IL-1β level in pg/mL was graphed in violin plots from Control and Heat Stress mouse serum samples (n = 3). All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. (F) Serum IL-6 level in pg/mL was graphed in violin plots from Control and Heat Stress mouse serum samples (n = 3). All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. (G) CD68 and IL-1β immunoreactivities were shown by immunohistochemistry in liver sections from Control and Heat Stress mouse groups; Images were taken at 20X magnification and displayed with a scale of 100 μm. (H) CD68 immunoreactivity in both groups was measured as arbitrary light units from six separate microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05. (I) IL-1β immunoreactivity in both groups was measured as arbitrary light units from six separate microscopic fields and plotted along the ordinate. All p values were derived through an unpaired t-test, with the significance level set at p < 0.05.