Figure 2

Heat stress-induced transcriptional alterations in the brain. (A) Volcano plot obtained from comparing the brain tissues of control and heat stress groups that were analyzed for differential gene expression changes through transcriptomic profiling, -Log₁₀ P (P_FDR) and Log₂fold changes were plotted respectively along the ordinate and abscissa with a significance cut off set at P_FDR < 0.05, Log₂ fold change > 1. The blue dots represented downregulated, and the red dots represented the upregulated genes. Genes represented in gray dots were laid in the zone of no significance. The total number of upregulated and downregulated genes was indicated with similar color arrowheads. (B) The heatmap generated from the brain tissue volcano plot was delineated with a Log₂ fold change gradient between 1.5 and − 1.5 where the extremities of red and blue signified up- and downregulations respectively. The same blue and red color code was set to represent the intensity of gene expression falling within the given gradient reflecting the Log₂ fold change of individual genes named parallel.