Table1. Descriptions of the primer sequences used for the qRT-PCR reactions.
From: Cellular mechanisms mediating the anti-cancer effects of carnosol on gingiva carcinoma
Gene | Primer sequence (5’–3’) | Amplicon size (bp) | Tm (°C) |
|---|---|---|---|
IL-6 | Fw: 5'-TCTCCACAAGCGCCTTCG-3’ Rv: 5'-CTCAGGGCTGAGATGCCG-3’ | 203 | 60 |
MMP-9 | Fw: 5'-ATTTCTGCAGCTCTGTGTGAA-3’Rv: 5'-TGAATTCTCAGCCCTCTTCAA-3’ | 263 | 60 |
TIMP-1 | Fw:5′-ATCCTGTTGTTGCTGTGGCTGATAG-3′Rv:5′-TGCTGGGTTAACTCTTTATTTCA3′ | 689 | 58 |
GAPDH | Fw:5'GGTATCGTCGAAGGACTCATGAC-3’Rv:5'-ATGCCATGAGCTTCCCGTTCAGC-3’ | 180 | 60 |
