Figure 4
From: Development of a new caged intein for multi-input conditional translation of synthetic mRNA
Orthogonality check of the caged Rma DnaB and Npu DnaE inteins by translational repression using EGFP-responsive C46-split CaVT. (a) Schematic depiction of mRNAs used in EGFP-responsive translational repression via caged Npu DnaE intein. (b) HeLa cells were co-transfected with 2xScMS2(C)-Luc2 (10 ng/well), Nluc (1 ng/well), and two types of mRNAs to express split CaVT per group, (40 ng/well each), and EGFP or eDHFR (10 ng/well) mRNAs. MS2CP(1–45)-eNpuNcage-Lag16 with GFPenhNb-NpuCcage-MS2CP(46–116)-VPg(FCV) group and MS2CP(1–45)-RmaN(-4)cage-Lag16 with GFPenhNb-RmaC-MS2CP(46–116)-VPg(FCV) group were used as positive controls for EGFP-responsive translational repression. The bar graph shows the Luc2/Nluc ratio (mean ± SD, n = 4). **P < 0.01, ***P < 0.001 compared with eDHFR-transfected (EGFP-untransfected) cells in each group by the unpaired two-sided Student’s t-test.
