Figure 2 | Scientific Reports

Figure 2

From: Assessing the combined impact of fatty liver-induced TGF-β1 and LPS-activated macrophages in fibrosis through a novel 3D serial section methodology

Figure 2

(A) Azan staining of liver sections. Liver sections were prepared from mice treated as shown in Fig. 1A. The fibrous connective tissues in each specimen were stained blue (arrow heads). Asterisks indicate ballooning hepatocytes. HFD, high-fat diet; ND, normal diet. (B) Immunohistochemical staining of liver sections. Liver sections were prepared as indicated in (A), and then immunohistochemical staining using anti-collagen type 1a (Col1a) antibody was performed. 3,3′-Diaminobenzidine (DAB) was used to detect the Col1a signals (brown: arrow heads). Nuclei stained with hematoxylin (blue).

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