Table 3 Primers used in gene expression analysis of the interaction between cassava genotypes and pathogens associated with root rot at different times.
GENE ID | Forward primer sequence (5´- 3´) | GC% | Reverse primer sequence (5´- 3´) | GC% | Amplicon length (pb) |
|---|---|---|---|---|---|
MANES_01G170600 | TTGCAGACAGGAGCTGCCCC | 65% | CCCTGGCTTCACCTTCAGCC | 65% | 114 |
MANES_01G250800 | CGCAGCAGGGGAACCAAATG | 60% | GCGGAAATAGGGGTAGCACG | 60% | 164 |
MANES_02G109100 | TAGCTCACCTCGGCATCCTG | 60% | AATTTGTGTCCACGGCGAGG | 55% | 118 |
MANES_03G129300 | GCATTAGGAGGGGCTGCAGT | 60% | CTCGCCATCAGCAGCTGATT | 55% | 147 |
MANES_07G008700 | AGATCGGATTGTGGTGGGGC | 60% | CGCCTTAGAGCTTCAGTGGC | 60% | 199 |
MANES_08G043700 | GGCAGCACTACTACTGGTGC | 60% | CGGGGATCGGATTGAAGGGC | 65% | 126 |
MANES_08G130900 | CTCTGCCTTTGACCCTAGCC | 60% | GGTTCTTGCAGAACTTCCCG | 55% | 95 |
MANES_09G064600 | CGGCGGACCATATACGGAGC | 65% | CGCCACCCAGTCAAGAGCCT | 65% | 117 |
MANES_09G146400 | GGGCAGATGCTGCGGGATTG | 65% | TCTACTCGGTTGGCCACCCA | 60% | 110 |
MANES_10G106500 | AGAGGGAGACCCTCCAATCC | 60% | CCACCAAGGAGGGCTAAGAT | 55% | 212 |
MANES_11G005800 | GCGAGTTGGGAACGCGACGG | 70% | CATGGCCAGGCATGTCGTGC | 65% | 169 |
MANES_11G121200 | TGTTGAGAGTGTGCAGGCGC | 60% | GCTCAGCCAGCTCATCACCA | 60% | 176 |
MANES_11G165400 | ATCTTGCTGCTGCTCTGGGC | 60% | CCCCGTCGACAATGCCATGG | 65% | 177 |
MANES_12G144000 | TTCCCACCAGGTCCTCGAGC | 65% | GGTTCAAAGCCCATGCGCAA | 55% | 134 |
MANES_13G135900 | CTCCCCGGAGCTCCGTTCGA | 70% | CCGGTTCCCTCTCCGGCCTA | 70% | 149 |
MANES_14G131900 | GCACCGGCCTCTTCCTGACA | 65% | GCTAACCATCCCTGGCCACC | 65% | 109 |
MANES_15G004100 | GGCAGCTCAGCTAGTAGGCC | 65% | CGCCTCCTTCGGATTGACGG | 65% | 137 |
MANES_15G130600 | GCGAATGGGGGAAAGAAGGC | 60% | GCACCAAGCTTCCATCCCCC | 65% | 204 |
