Figure 3
From: Pre-analytical challenges from adsorptive losses associated with thiamine analysis
Concentration of thiamine recovered following storage of 1 mL 100 nM thiamine in deionized water for 3 h at 4 °C or 21 °C in 8 × 40 mm 1 mL amber glass and polypropylene autosampler vials, 1.5 mL clear non-silanized and silanized glass autosampler vials, polypropylene 1.5 mL centrifuge tubes, polystyrene 5-mL culture tubes, glass culture tubes (10 × 75 mm) as compared to a 50 mL stock solution stored in a 50-mL polypropylene tube. A vertical dashed line separates materials that may be directly compared based on their surface-to-volume ratios. The results are after conversion of the thiamine remaining in the solution to thiochrome using alkaline ferricyanide with fluorescence detection at λex = 360/40 nm, λem = 450/50 nm.
