Figure 7
From: Pre-analytical challenges from adsorptive losses associated with thiamine analysis
Concentration of thiamine recovered following filtration of 125 mL 100 nM thiamine in commercially bottled HPLC grade water pH adjusted to 3.5–6.5 through (a) GF/F glass fiber (0.7 μm pore size) and (b) 0.2 µm polyethersulfone (PES) filters after vortexing filters in 2 mL alkaline potassium ferricyanide. The results are after conversion of the filter-retained thiamine to thiochrome using alkaline ferricyanide with fluorescence detection at (a) λex = 360/40 nm, λem = 450/50 nm and (b) λex = 360/9 nm, λem = 450/15 nm.
