Table 3 Antibodies used in this immunohistochemical study.
From: Follicle-stimulating hormone receptor expression in advanced atherosclerotic plaques
Antibody | Specificity | Description | Isotype | Antigen retrieval | Dilution | Incubation (min) | Supplier |
|---|---|---|---|---|---|---|---|
FSHR1-A02 | Human FSH-R1 | Mouse monoclonal | IgG2a | Citrate buffer pH 6 | 0.2 µg/ml | 30 | FSHR Theranostics |
F3520 | Human vWF | Rabbit polyclonal | Citrate buffer pH 6 | 1 to 3000 | 30 | Sigma | |
M7165 Qbend | CD34 | Mouse monoclonal | IgG1 | Tris EDTA pH 9 | 1 to 200 | 30 | Dako |
M3619, clone D2-40 | Podoplanin | Mouse monoclonal | IgG1 | Citrate buffer pH 6 | 1 to 100 | 30 | Dako |
KP1 | CD68 | Mouse monoclonal | IgG1 | Tris EDTA, pH 9 | 1 to 500 | 30 | Dako |
NCL L-163 clone 10D6 | CD163 | Mouse monoclonal | IgG1 | Tris EDTA, pH 9 | 1 to 500 | 30 | Dako |
M0851 clone | Alpha SMC actin | Mouse monoclonal | IgG1 | Tris EDTA, pH 9 | 1 to 200 | 30 | Dako |
M9144 | Irrelevant antigen | Mouse monoclonal | IgG2a | Citrate buffer pH 6 | 0.2 µg/ml | 30 | Sigma |
