Table 2 The selected HTL epitopes for the final vaccine construction are provided by the NetMHC II pan 3.2 and IEDB server.
Proteins/epitopes (position) | HLA class II alleles | Antigenicity/allergenicity | Toxicity | Epitope conservation (%) | Homology to human proteins |
|---|---|---|---|---|---|
Spike/GINITRFQTLLALHR (232–246) | HLA-DRB5*01:01, HLA-DRB1*04:04, HLA-DRB1*01:01, HLA-DRB1*11:01, HLA-DRB1*04:05, HLA-DRB1*04:01, HLA-DRB1*15:01, HLA-DRB4*01:01, HLA-DRB1*07:01, HLA-DRB1*09:01, HLA-DPA1*01:03/DPB1*02:01, HLA-DPA1*03:01/DPB1*04:02, HLA-DPA1*02:01/DPB1*01:01, HLA-DPA1*01/DPB1*04:01 | 0.5582 (antigen)/non-allergen | Non-toxin | 100% | Non-homolog |
Spike/TNSRRRARSVASQSI (676–690) | HLA-DRB1*07:01, HLA-DRB1*01:01 | 0.7267 (antigen)/non-allergen | Non-toxin | 100% | Non-homolog |
Spike/DYSVLYNLAPFFTFK (361–375) | HLA-DRB1*01:01, HLA-DRB1*04:04, HLA-DRB1*08:03, HLA-DRB1*12:01, HLA-DRB1*13:02, HLA-DRB3*02:02, HLA-DRB3*01:01, HLA-DPA1*01:03/DPB1*04:01, HLA-DPA1*01:03/DPB1*04:02, HLA-DPA1*01,03/DPB1*23:01, HLA-DQA1*01:02/DQB1*06:04, HLA-DPA1*01:03/DPB1*02:01 | 0.7838 (antigen)/non-allergen | Non-toxin | 100% | Non-homolog |
Nucleocapsid/ALALLLLDRLNQLES (218–232) | HLA-DRB4*01:01, HLA-DRB1*03:01, HLA-DRB1*11:01, HLA-DRB1*04:04, HLA-DRB1*01:01, HLA-DPA1*03:01/DPB1*04:02, HLA-DPA1*01:03/DPB1*02:01, HLA-DPA1*02:01/DPB1*01:01 | 0.5057 (antigen)/non-allergen | Non-toxin | 100% | Non-homolog |
Membrane/VGLMWLSYFIASFRL (88–102) | HLA-DRB5*01:01, HLA-DRB1*04:04, HLA-DRB1*01:01, HLA-DRB1*15:01, HLA-DRB1*04:05, HLA-DRB1*07:01, HLA-DRB1*04:01, HLA-DPA1*01:03/DPB1*02:01, HLA-DPA1*02:01/DPB1*01:01, HLA-DPA1*01/DPB1*04:01 | 0.6658 (antigen)/non-allergen | Non-toxin | 100% | Non-homolog |
Envelope/FYVYSRVKNLNSSRV (56–70) | HLA-DRB1*01:01, HLA-DRB1*04:04, HLA-DRB1*07:01, HLA-DRB1*11:01, HLA-DRB1*04:01, HLA-DRB1*04:05, HLA-DRB1*09:01, HLA-DRB1*13:02, HLA-DRB1*15:01 | 0.6103 (antigen)/non-allergen | Non-toxin | 100% | Non-homolog |
