Figure 1

Inhibition of dengue virus type 2 (DENV2) production by pimecrolimus, ascomycin, and tacrolimus in Vero cells,Vero cells were infected with DENV2 at 0.1 multiplicity of infection (MOI) and treated with 20 μΜ pimecrolimus, ascomycin, or tacrolimus and 500 μΜ ribavirin. (a) Relative DENV2 genome expression across the various treatments was determined via real-time PCR at different time points. (b) Culture supernatants at different time points were used to infect BHK-21 cells. DENV2 titers were determined using plaque assays. Data are expressed as mean ± SEM (n = 3 independent biological experiments, each conducted with triplicate technical repeats).