Figure 4

Pimecrolimus limits DENV2 localization in Vero cells, (a) Immunofluorescence images of Vero cells infected with 0.1 MOI of DENV2 and treated with pimecrolimus or ribavirin at the indicated concentrations for 48 h. The expression of the DENV envelope (DENV E) protein was used to distinguish DENV2-positive cells. Nuclei are stained with DAPI. Scale bar: 50 μm. (b) Huh-7 cells infected with 0.1 MOI of DENV2 for 72 h at the indicated concentrations of pimecrolimus or ribavirin. The protein levels of DENV E were determined via western blotting. The expression of GAPDH was used as a loading control. (c) Quantification of the DENV E fold-change in protein levels relative to the DENV2 and vehicle-treated group. Data are expressed as mean ± SEM (n = 3 independent biological experiments, each conducted with triplicate technical repeats; ns, not significant; ***p < 0.001; comparisons made against the DENV2-infected and vehicle-treated group).