Figure 4
From: Molecular histopathology of matrix proteins through autofluorescence super-resolution microscopy
Quantification of broad-field MUSI-tAF features in delineating human oral pre-cancer stages. Ratio of (a) epifluorescence intensity and (b–e) MUSI-tAF intensity between the layers of epithelium and sub-epithelium to delineate the stages-NOM, OSF, OSFD, and OSCC. The used abbreviations can be found in Fig. 3. N = 60–100 per group (patient samples = 5 \(\times\) ROI = 20). (f–k) Variance quantification of different layers and sub-layers of epithelium and sub-epithelium to delineate the oral pre-cancer stages. In OSCC tissues, the epithelium and sub-epithelium cannot be differentiated due to metastasis and hence were not included in (b,d,f,g,i,j). N = 30–60 per group (patient samples = 5 \(\times\) ROI = 10) , All plots are represented as \(\mu\) ± SD, ns (not significant) p > 0.05, *p \(\le\) 0.05, **p \(\le\) 0.01, ***p \(\le\) 0.001, ****p \(\le\) 0.0001. For statistical analysis, we used one-way ANOVA (95% confidence interval) followed by Tukey’s post hoc test.
