Figure 5
From: Molecular histopathology of matrix proteins through autofluorescence super-resolution microscopy
MUSI-tAF in detecting keratin-associated human oral leukoplakia. (a) Histology of tissue with leukoplakia, (b) pan-CK (keratin) stained region of interest to show the distribution of cytokeratin in the tissue, (c,d) are MUSI-tAF images showing different regions in the tissue. (e) Side-by-side view of epifluorescence and super-resolved image, (f) another region showing pan-CK distribution, (g) extracted image after color deconvolution containing only keratin density information, (h) is the region taken from the papillary region showing the distribution of keratin with MUSI-tAF, (i,j) Intensities of MUSI-tAF and keratin stain showing similar ratios of intensities. N = 100 per group (patient samples = 5 \(\times\) ROI = 20), All plots are represented as \(\mu\) ± SD, ****p \(\le\) 0.0001. For statistical analysis, we used a two-tailed t-test (95% confidence interval).
