Figure 1 | Scientific Reports

Figure 1

From: APEX2-based proximity proteomic analysis identifies candidate interactors for Plasmodium falciparum knob-associated histidine-rich protein in infected erythrocytes

Figure 1

CRISPR/Cas9 guided knock-in scheme to tag the Plasmodium falciparum kahrp gene with APEX2. (A) A schematic figure illustrating integration of the cassette from the pCC1-KAHRP-FLAG-APEX2 vector into parasite gDNA, resulting in integration of the FLAG tag and APEX2 domain at the 3′ end of the gene, in frame with the KAHRP ORF. (B) PCR validation of cassette integration. Legend: K (KAHRP-FLAG-APEX2) and C- (negative control).

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