Figure 1

Location of the study sites along a linear urban–rural gradient from Berlin city into Brandenburg. Along this gradient, six distance classes at 5 km intervals were defined to reflect the decreasing urbanization level. Within each distance class, we established 3 sampling sites, which were monitored once per week between April and October 2020 and 2021 and were considered to be independent. Arrows indicate the westwards directed urban–rural gradient and define x-axes in subsequent figures, (I–VI). Distance classes I–II: urban; distance class III: suburban; distance class IV–V: peri-urban; distance class VI: rural. The external borders of the district of Berlin and the federal state of Brandenburg were created on the basis of this file: https://en.wikipedia.org/wiki/States_of_Germany#/media/File:Germany_location_map.svg (NordNordWest, CC BY-SA 3.0); the rest of the figure was hand drawn by one of the authors (N.S.).