Figure 2

Schematic illustration of the measurement with single-cell resolution optical sensor. The cells were added to 12 wells in the microplate and measured (A). The sensor can detect refractive index change above the surface in an approximately 150 nm thick layer, which corresponds to the evanescent field [(B), ‘side view’ part, red zone]. The integrin-ligand binding happens in this layer, thus the sensor can monitor cell adhesion and provides kinetic data of the process (C).