Table 1 Candidate reference genes, primer sequences and amplicon characteristics of the lotus.
Gene symbol | Gene name | Gene accession | Sequence of forward & reverse primers | Amplicon length (bp) | R2 | Primer efficiency |
|---|---|---|---|---|---|---|
18S | 18S ribosomal RNA | XM-010264930.1 | AGTATCTCGGCAGTTCAGTG | 191 | 0.997 | 92.827 |
GCAAATCCAGCACGCATA | ||||||
ACT | β-actin | XM-010243420.1 | TCGGTTGGACCTTGCT | 207 | 0.996 | 100.353 |
CCATCAGGCAGCTCGTA | ||||||
CYP | Cyclophilin | XM-010245789.1 | GTACCCAGAAGAATGCCCTA | 102 | 0.998 | 96.222 |
ATGAAGCCCTTGATGACTCG | ||||||
UBQ | Ubiquitin | XM-010264087.1 | TCGCACCTTGGCTGACT | 281 | 0.996 | 95.465 |
GGACAAGATGAAGGGTGG | ||||||
UBC | Ubiquitin-conjugating enzyme | XM-010246251.1 | AACATCAATAGCAATGGGAGC | 116 | 0.997 | 97.752 |
GGATTTGGGTCCGTTAG | ||||||
TUA | Tubulin alpha | XM-010268491.1 | GTCTTCTCCCGAATTGACCAC | 86 | 0.998 | 95.173 |
TCTTCCATGCCCTCGCCAA | ||||||
GAPDHHHHHHHH | Glyceraldehyde-3-phos -hate dehydrogenase | XM-010250569.1 | CGAACAGATCAAAGCCGCTA | 199 | 0.997 | 94.249 |
GCTGTAACCCCATTCGTTG | ||||||
EF-1α | Eukaryotic elongation factor | XM-010246657.1 | CAAGTCTGTTGAGATGCACCA | 119 | 0.999 | 88.998 |
AGTTCGAGGCAACATAACCAC | ||||||
MDH | Malate dehydrogenase | XM-010264019.1 | CCATAACAGGGCACTAGGTCA | 164 | 0.999 | 94.338 |
CAACAAGCTCACGTACAGG | ||||||
PLA | Phospholipase A2 | XM-010268730.1 | TTCTCCTGTGTCCTCCTCGTC | 120 | 0.997 | 91.09 |
TACTCCACAGAACTTCCCGTA | ||||||
TBP | TATA-box-binding protein | XM-010278104.1 | TTTCCAGCAAAGTTTAAGGAC | 101 | 0.997 | 98.549 |
GCACCATGAGAATATGCAAG | ||||||
Eif-5a | Eukaryotic initiation factor 5a | XM-010267296.1 | GCCTTCGTCCCACAACT | 94 | 0.998 | 90.252 |
AGTCAGAAGGCTCACAA |
