Figure 5 | Scientific Reports

Figure 5

From: The influence of endurance exercise training on myocardial fibrosis and arrhythmogenesis in a coxsackievirus B3 myocarditis mouse model

Figure 5

Cardiac PCR in the continued EEX study. (A) Cardiac CVB viral load at sacrifice as determined by dPCR. The viral load per group is expressed as copies per ng of RNA. A Mann–Whitney U test did not detect a significant difference between the CVB-EEX and CVB-SED group (P = 0.210). Group size: n = 4 for PBS groups and n = 6 for CVB groups. The mean myocardial viral load for similar animals (strain, age, sex, inoculation dose) at 7 days after inoculation is shown for reference. (B-F) Cardiac gene expression at sacrifice as determined by qPCR. Messenger RNA levels are expressed as the fold change (FC) over the PBS-SED control group. Potential differences in CTGF, TGF-β and COL3A1 gene expression were evaluated via one-way ANOVA with post hoc Tukey’s test. For comparison of MMP-9 and COL1A1 gene expression between groups, the Kruskal–Wallis test was used, followed by Dunn’s multiple comparisons test. Group size: PBS-SED: n = 14; PBS-EEX: n = 14; CVB-SED: n = 16; CVB-EEX: n = 16.

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