Figure 3

Data-analysis workflow with intermediary images. Demonstrates the data analysis workflow through representative datasets. (a) MSI of the ion present at m/z 885.55. (b) PC1 projection used for coregistration. (c) H&E staining with annotations and extraction mask. Red: Tumor, Green: Non-tumor, Blue: Background. (d) Coregistration of H&E image and PC1. Grey: pixels found in both images, Black: pixels exclusively found in MSI, White: pixels exclusively found in H&E image. (e) Projection of model classification. Red: Tumor; Green: Non-tumor; Black: Tissue boundary; White: Misclassifications according to the validating pathologist with Tumor 232 showing an example of false positive prediction and Tumor 254 showing an example of false negative prediction.