Figure 3 | Scientific Reports

Figure 3

From: Use of phase plate cryo-EM reveals conformation diversity of therapeutic IgG with 50 kDa Fab fragment resolved below 6 Å

Figure 3

Phase plate cryo-EM imaging of DDM-embedded Rituximab. (A) ZPP images show Rituximab (~ 0.5 mg/ml with 0.008% DDM) molecules are sparsely distributed as denoted in red circles. (B) Four representative 2D class averages generated from picked ZPP Rituximab particle images. (C) VPP images of Rituximab (~ 2.0 mg/ml with 0.008% DDM). (D) 2D class averages generated from picked VPP Rituximab particle images. (E) Representative 2D re-projections from an IgG crystal structure (PDB: 1IGT) for comparison.

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