Figure 4 | Scientific Reports

Figure 4

From: Use of phase plate cryo-EM reveals conformation diversity of therapeutic IgG with 50 kDa Fab fragment resolved below 6 Å

Figure 4

3D reconstruction of Rituximab from Volta phase plate (VPP) cryo-EM images. (A) 3D cryo-EM reconstruction of Rituximab with Fab density further focused refined as shown in the box. (B–D) Angular distribution of IgG particle orientations, FSC from cryoSparc for resolution estimation, and local resolution maps. (E) Docking of Fab and Fc as individual domains into our cryo-EM reconstruction. (F) Comparison of the pseudo atomic model generated from (E) to that of a crystal structure (PDB: 1IGT).

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