Table 1 Cryo-EM used for IgG studies.
Sample name | Rituximab (full length antibody) | ||
|---|---|---|---|
EM equipment | JEM-2200FS | JEM-F200 | TALOS Artica |
Phase plate engaged | Yes | Yes | Yes |
Voltage (kV) | 200 | 200 | 200 |
Detector | DE-20 | K2 | Falcon3 |
Magnification | 40,000× | 40,000× | 120,000× |
Pixel size (Å/pixel) (Imaging) | 1.4 | 1.09 | 0.855 |
Pixel size (Å/pixel) (Processing) | 1.4 | 1.09 | 0.855 |
Electron exposure (e–/Å2) | 42 | 50 | 50 |
Exposure time (s) | 3 | 8 | 2.5 |
Frames (no.) | 75 | 40 | 50 |
Defocus range (μm) | − 0.0 to − 0.5 | − 0.5 to − 0.8 | − 0.5 to − 0.8 |
Micrographs stacks (no.) | 107 | 3 | 606 |
Picking particle images (no.) | 22,979 | N.A | 173,336 |
Final particle images (no.) | 1305 | N.A | 31,111 |
