Figure 2
Cell proliferation and viability of OA chondrocytes. (A) Control chondrocytes and OA chondrocytes were cultured in vitro for 2 h, 6 h, 12 h, 24 h and 48 h and cell proliferation was detected by MTT. (B) OA chondrocytes were induced by different treatments and cell proliferation was detected by MTT. (C) Observation of cell state. (D) Crystal violet staining. (E) Control chondrocytes and OA chondrocytes were cultured in vitro for 24 h and dyed by Calcein AM/PI. (F) Quantification of fluorescence intensity. Results are presented as means ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus Con. #P < 0.05, ##P < 0.01 versus OA.
