Figure 3
Cell apoptosis of OA chondrocytes. (A) Control chondrocytes and OA chondrocytes were cultured in vitro for 2 h, 6 h, 12 h, 24 h and 48 h. (C) OA chondrocytes were induced by different treatments. FITC annexin V/PI staining and flow cytometry assays were used to detect cell apoptosis. Control chondrocytes cultured in DMEM were used as control. (B) and (D) Quantification of apoptosis in different groups. Results are presented as means ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus Con. ##P < 0.01 versus OA.
