Figure 5
Expressions of relative genes and proteins. (A) Control chondrocytes and treated OA chondrocytes were harvested and total RNA was extracted, followed by qRT-PCR for detection of aggrecan, Col2a1, iNOS, PPARγ, caspase-9 and caspase-3gene expression levels. (B) Control chondrocytes and treated OA chondrocytes were harvested and total protein was extracted, western blotting was used to detect the expression of aggrecan, Collage II, iNOS, PPARγ, caspase-9 and caspase-3. (C) Quantification data of western blotting. The relative protein levels were normalized to the level of the internal control, β-Actin and presented as fold changes relative to the control group (the level of the control group was set as 1). Results are presented as means ± standard deviation of three independent experiments. **P < 0.01, ***P < 0.001 versus Con. #P < 0.05, ##P < 0.01, ###P < 0.001 versus OA.
