Table 4 Effect of plant growth regulators on hypocotyl callus induction and differentiation.
From: Establishment of an efficient regeneration system of ‘ZiKui’ tea with hypocotyl as explants
Processing condition | Inoculated number | Callus induction rate (%) | Callus number | Induction rate of bud differentiation (%) | Number of differentiated buds | Callus growth status |
|---|---|---|---|---|---|---|
WPM + 2.00 mg/L 6-BA + 0.10 mg/L NAA | 90 | 95.19 ± 0.64a | 86 | 20.74 ± 0.64a | 19 | Green, compact structure, many adventitious buds |
WPM + 2.00 mg/L 6-BA + 0.30 mg/L NAA | 90 | 96.30 ± 1.70a | 87 | 15.93 ± 1.70c | 14 | Green, compact structure, few adventitious buds |
WPM + 2.00 mg/L 6-BA + 0.10 mg/L IBA | 90 | 91.85 ± 1.28b | 83 | 19.03 ± 1.11ab | 17 | Yellow-green, covering the surface of the cotyl, compact structure, few adventitious buds |
WPM + 2.00 mg/L 6-BA + 0.30 mg/L IBA | 90 | 97.04 ± 1.70a | 88 | 18.15 ± 1.70b | 16 | Yellow-green, covering the surface of the cotyl, compact structure, few adventitious buds |
WPM + 2.00 mg/L 6-BA + 0.10 mg/L 2, 4-D | 90 | 90.00 ± 1.11bc | 81 | 15.56 ± 1.11c | 14 | White and light green, covering the cotyl indicates a loose structure with few adventitious buds |
WPM + 2.00 mg/L 6-BA + 0.30 mg/L 2, 4-D | 90 | 88.89 ± 2.22c | 80 | 8.52 ± 0.64d | 6 | White and light green, covering the cotyl indicates a loose structure with few adventitious buds |
