Figure 5
Functional complementation of THI6∆ (deficient in thiamin monophosphate synthase) and THI20/21∆ (deficient in HMP-P kinase) yeast mutants. The mutants transformed with the empty vector (EV) were used as negative control with no exogenous supply of thiamin (0) (THI20/21∆_EV_0 and THI6∆_EV_0), while the wild type transformed with the empty vector (EV) and no added thiamin (WT_EV_O) was used as positive control for all experiments. In all cases, THI20/21∆_OsTH1_0 and THI6∆_OsTH1_0 represent the mutants transformed with OsTH1 with no added thiamin while THI20/21∆_EV_A, THI6∆_EV_A, THI20/21∆_EV_B and THI6∆_EV_B represent the complementation of the mutant with addition of 1μM thiamin (A) or 2 μM thiamin (B). (a) Cell spotting for THI20/21∆, deficient in HMP-kinase. (b) Cell spotting for THI6∆, deficient in TMP synthase. (c) Growth curve and growth rate of THI20/21∆, deficient in HMP-kinase. (d) Growth curve and growth rate of THI6∆, deficient in HMP-kinase. Data shown correspond to means ± standard errors of the means (SEM). Data was compared to control values (WT) by One-way ANOVA followed by Tukey’s post hoc test (****, P < 0.0001).
