Figure 1

Heatmap and barplot of the PIK3CA mutations and subsequent information of the cohorts’ primary and relapse tumors. Exon location, mutation variants, Combined Annotation-Dependent Depletion (CADD) score evaluation (yellow = CADD-score equal to or above 20, green = CADD-score below 20), status of co-occurrence with other mutations (light-blue = co-occurs with other PIK3CA mutations, light-purple = does not co-occur with other PIK3CA mutations), hotspot status (red = hotspot mutation, blue non-hotspot mutation), and variant allele frequency (VAF) comparisons on X-axis (number of patients in which the VAF is higher in either tumor sample; purple = VAF higher in primary tumor than relapse tumor, green = VAF higher in relapse tumor than primary tumor) for each of the individual mutations found. NA = comparison not possible (left blank), orphan samples.