Figure 9

DHODH knockout attenuates the proliferation, migration, and ferroptosis of 786-O and OS-RC-2 cells. (A) Knockdown of DHODH in 786-O and OS-RC-2 cells and evaluation of the cell proliferation ability using the EDU assay. (B) Western Blotting was performed to determine the expression of EMT-related proteins in 786-O and OS-RC-2 cells transfected with DHODH or the control for 48 h. (C) Western blot analysis was performed to determine the expression of ferroptosis-related proteins in 786-O and OS-RC-2 cells after knockdown of DHODH. (D) Western blot analysis was performed to determine the expression of the apoptotic protein, Parp-1, after DHODH knockdown. (E) Expression of ferroptosis-related protein in ccRCC tissues and normal renal tissues. (F) Immunohistochemistry was performed to visualize the expression of ferroptosis-associated protein in normal renal tissue and renal clear cell carcinoma. (G) Changes in the mitochondria of renal clear cancer cells after DHODH knock down via transmission electron microscopy. Scale bar represents 1 µm (× 12000). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. The asterisk represents the degree of statistically significant difference (n = 3).