Figure 10 | Scientific Reports

Figure 10

From: Integration of single-cell sequencing and bulk RNA-seq to identify and develop a prognostic signature related to colorectal cancer stem cells

Figure 10

Validation of CRCSC-related genes by qRT-PCR. (A) Scatterplot identifies mtry = 4 as the optimal parameter for constructing the Random Forest model. (B) Random forest analysis highlights RPS17, TIMP1, ALDH2, FDFT1, PSMG3 and PSMA5 as key genes with stable contribution scores and significant model importance. (C,D) Morphological evidence of CSC enrichment in DLD-1 and HCT116 cells following a 7-day enrichment protocol. (E,F) qRT-PCR validation of increased ALDH1A1 expression in enriched CRCSCs (P < 0.05). (G,H) qRT-PCR validation of elevated NOTCH expression in enriched CRCSCs (P < 0.05).

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