Figure 11

Experimental and mechanistic prediction of RPS17 expression in CRC. (A) qRT-PCR confirmed the high expression of RPS17 in CRC cell lines compared to normal colorectal epithelial cells, NCM460 for normal colorectal epithelial cells, and DLD-1, HCT116, HCT15, HT-29 and SW620 for CRC group cell lines, P < 0.05. (B) Western blot immunoblotting showed significantly elevated protein levels of RPS17 in CRC cells. (C) Western blot immunoblot statistical analysis plots with one-way ANOVA. (D) Differential analysis demonstrating that RPS17 is highly expressed in CRC. (E) Pairwise differential expression demonstrating that RPS17 is highly expressed in CRC. (F) Survival differential analysis demonstrating that the overall survival rate of patients with high expression of RPS17 is significantly lower than that of patients with low expression. (G) GO results show that RPS17 is enriched in DNA packaging during biological processes, and that cellular components are enriched in collagen-containing extracellular matrix and are active in the molecular function of protein heterodimerization activity, corresponding to the circle diagrams demonstrated in the (G). (H) Violin diagrams demonstrates that high expression of RPS17 leads to a decrease in both TME scores (including Stromal Score, Immune Score, ESTIMATE Score).