Figure 4 | Scientific Reports

Figure 4

From: Integration of single-cell sequencing and bulk RNA-seq to identify and develop a prognostic signature related to colorectal cancer stem cells

Figure 4

CRCSCs differential gene screening and survival significance study. (A) 1158 colorectal CSCs differential genes from scRNA-seq data for volcano plot visualization. (B) Scatterplot of GO functional analysis of differential genes. (C) Forest plot demonstrating the screening of 26 prognostically relevant genes after univariate COX regression scores (P < 0.05). Among the 26 genes associated with prognosis, 20 genes that were differentially expressed in CRC were extracted and shown as (D) box plots and (E) heat maps, respectively. (P < 0.05). (F) Prognosis-related consensus clustering matrix at K = 2. (G) Relative changes in the area under the CDF curves at K = 2–9. (H) Empirical CDF plots at K = 2–9. (I) Survival difference analysis between Cluster1 and Cluster2 (P < 0.05).

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