Figure 1

Correlation of combined tumor purity to protein abundance in PDS. (a) Bar chart of both RNA- (n = 21) and DNA- (n = 24) based purity calculation for PDS cases and the top 50, most correlating proteins to tumor purity, by following a Spearman correlation of each protein with tumor purity. The bar length corresponds to a Spearman correlation coefficient of an individual protein (visualized as human genome organization gene symbol; row), the color is coded for the mean correlation between both RNA and DNA correlation to tumor purity. The scale bar visualizes the mean Spearman correlation coefficient. (b) Scatter plot of an Enrichr¹¹ analysis using the “ARCHS4 tissue” database of the top 50 proteins most correlating to tumor purity. The color code represents significant findings (FDR < 0.05, red) (n = 24). The adjusted p-value, the odds ratio and the log2 of the combined score (dot size) are quantitative and qualitative results of the Enrichr overrepresentation analysis, which briefly reflect the relevance of a given term in association to the proteins (here, we used corresponding HUGO symbols as input). (c) Heatmap of most highly expressed 10 genes, according to average gene expression within the single cell data¹², which were also among the top 50 genes most correlating to RNA/DNA tumor purity of panel A (each column represents one cell, total samples n = 5).