Figure 4
From: Expression and potential molecular mechanism of TOP2A in metastasis of non-small cell lung cancer
TOP2A is involved in the progression and development of NSCLC via the Wnt/β-catenin signaling cascade. (A) TCGA enrichment analysis revealed that TOP2A expression is enriched in the Wnt signaling pathway (n = 1037). (B) Several NSCLC cell lines exhibited a clear association between the transcription of TOP2A and β-catenin. (C) The online GEPIA exhibited a correlation between TOP2A and WNT3A gene expression levels. (D,E) TOP2A overexpression and knockdown groups were examined for gene and protein levels of TOP2A, Wnt3a, and β-catenin. (F) Immunohistochemical assessment of Wnt3a and β-catenin expression in the TOP2A-positive and TOP2A-negative groups (200×). (G) Patients with high Wnt3a overexpression had an abysmal survival rate. *P < 0.05, **P < 0.01, or ***P < 0.001. All experiments were repeated three times independently.
