Figure 4

Circ_0001671 serves as a sponge for miR-27b-3p. (A) Biotin circRNA pull-down assay using biotin-circ_0001671, compared to control biotin-NC. (B) RIP analysis using anti-Ago2 antibody followed by detection of circ_0001671 and miR-27b-3p. Values were normalized to background RIP levels detected by IgG isotype controls. (C) Co-localization of circ_0001671 and miR-27b-3p in PC3 cells was assessed by FISH. Scale bars are 100 μm. (D) The luciferase activity of PC3 cells was measured after co-transfection with either the wild-type (WT) or mutant (MUT) circ_0001671 luciferase reporter vector and miR-27b-3p mimics or control miR mimics.