Table 1 Composition of Viscous medium.
From: Use of Viscous medium to study anthelmintic drug action in Caenorhabditis elegans
Componenta | Viscous medium from Papkou et al. 2019 | Viscous Medium from this study |
|---|---|---|
S basal Base: 10 mM Potassium Phosphate Buffer pH 6.0, 100 mM NaCl | ✔ | ✔ |
0.8% Hydroxypropylmethylcellulose | ✔ | ✔ |
10 mM Potassium Citrate buffer pH 6.0 | ✔ | ✔ |
Trace Metal Solution (55 uM disodium EDTA, 24 uM FeSO4 ·7H2O, 10 uM MnCl2·4H2O, 10 uM ZnSO4·7H2O, 1 uM CuSO4·5H2O) | ✔ | |
3 mM Mg2SO4 | ✔ | ✔ |
3 mM CaCl2 | ✔ | ✔ |
5 µg/ml cholesterol | ✔ | ✔ |
