Figure 1
From: Dysregulated Wnt and NFAT signaling in a Parkinson’s disease LRRK2 G2019S knock-in model
Experimental design for in vivo analyisis of canonical Wnt and NFATc1 signaling changes in wild type, LRRK2 KO and LRRK2 G2019S KI mice over time. Mice were injected intracranially at P0 with lentivirus containing a construct composed of a RE site for either TCF/LEF or NFATc1, a gene encoding luciferase and a second gene encoding for GFP. Expressed luciferase is able to cleave luciferin as a substrate, producing a detectable bioluminescence signal (A). This bioluminescence was measured regularly over 28 weeks in wild type (WT), LRRK2 knockout (KO) and LRRK2 G2019S knock-in (KI) mice in vivo (B). After 28 weeks, post-mortem analysis revealed luciferase activity prominently in the brain, excluding the other organs (C). Luciferase kinetics was determined with an ideal measurement time frame, highlighted in dark blue, n = 3 (D). Relative luciferase activity is shown in WT mice over time with signal correction by the signal generated SFFV construct, used as positive control (E).
