Figure 10
From: Dysregulated Wnt and NFAT signaling in a Parkinson’s disease LRRK2 G2019S knock-in model
Stimulation of primary neuronal cultures from LRRK2 KO, LRRK2 G2019S KI and WT mice. Wnt (TCF/LEF) and NFATc1 signaling changes are presented under treated and untreated conditions in primary cultured cortical (A) and hippocampal cells (B) from LRRK2 KO (1), LRRK2 G2019S KI (2), and WT mice. Cells were transduced with a lentiviral biosensor displaying Wnt or NFATc1 signaling activity. Data is shown as mean relative luciferase activity ± SEM, normalized to untreated WT set to 100%. Bioluminescence was measured 24h after treatment. Statistical significance shown as ###P < 0.0005, ##P < 0.005, and #P < 0.05 was tested for the effect of LRRK2 genotype via 2-way ANOVA and Bonferroni’s multiple comparison test and the effect of treatment shown as ****P < 0.0001, ***P < 0.0005, **P < 0.005, and *P < 0.05 was tested via 2-way ANOVA and Turkey’s multiple comparison test with n = 5 independent cultures.
